The CIB Cell Culture Facility gives scientific and technical support both to groups of the CIB an to external groups.
From the spring of 2016 theGeneral Facilities of the Service are located in the basement floor, room S-29 (ext. 4396). In this laboratory users have access to laminar flow cabinets, CO2 incubators, centrifuges, microscopes, baths, etc.
A. Supervision of the CIB Cell Culture Facilities
- Control of users' access (record of authorized users).
- Technical advice to the users who require it.
- Collaboration in the elaboration / updating of the rules for the use of the cell culture rooms. Supervision of the fulfillment of these rules.
- Assignment of equipment and shifts of use / cleaning of these equipment
- Preventive maintenance/ cleaning of specific equipment etc.
B. Mycoplasma Testing and Elimination
The Service carries out tests for the detection of mycoplasma contaminations using two techniques:
- Co culturing the samples (cell line, supernatant, medium...) with the Vero cell line and subsequent staining with Hoechst 33258.
- Luminometric estimation of ATP´s increase caused by mycoplasma.
The Service carries out the mycoplasma elimination of contaminated cultures with specific antibiotics. It is a slow procedure and its success cannot be guaranteed. The price will depend on the antibiotic used, the duration of the treatment and the materials used.
C. Selection of Fetal Bovine Serum
The biological properties of commercial Fetal Bovine Serum (FBS) presents variations both among different suppliers and among different batches of the same supplier. The Service collaborates with CIB research groups in the periodic testing of different brands and FBS´s batches to select one in which different cell lines grow adequately. The selected FBS batch is maintained as a general reserve of the CIB being available to researchers at the CIB Store facility. The duration of this reserve is calculated for a period of 18 to 24 months.
D. Maintenance of stock of cell lines in liquid nitrogen
E. Others (consult availability in the Service).For example: Maintenance in culture of cell lines, growth of hybridomas and obtaining supernatants.
F. Bioenergetic Characterization of Cell Lines
The CIB has an "Extracellular Flux Analyzer XF24-3" (Seahorse Bioscience), an instrument that allows a non-invasive characterization of the energy metabolism of cell lines in real time. The XF24-3 determines, in 24-well plates, the oxygen consumption (OCR), CO2 formation (CDER) and the extracellular acidification rate (ECAR, a proxy for the rate of lactate formation). External and CIB users have access to this equipment. Help with the design, running of the experiments or data analysis is provided by Dr. E. Rial in charge of the technical and scientific assistance.
Services and Rates
EXTERNAL USERS (RESEARCHERS FROM OTHER CENTERS / COMPANIES)
BIOENERGETIC: SERVICE MODES AND RATES (Request Form, Información, Modalidades de servicio y Tarifas, Tarifas usuarios CIB, Tarifas usuarios CSIC y UCM, Tarifas usuarios OPIs)