The Flow Cytometry Service is equipped with the following falicities:

 

1-ANALYZERS

 

-FC500-MPL (Beckman-Coulter) equipped with two blue and red emission lasers respectively. It can process six simultaneous signals: two scattering (forward and orthogonal) and four fluorescence (ranging between 525 to 675 nm).

 

-Cytfolex-S (Beckman-Coulter). equipped with four lasers (violet, blue, yellow-green and red). This analyzert can process fifteen simultaneous signals: two scattering and thirteen fluorescence (ranging between 450 to 800 nm )

 

2-Cell Sorter: FACSAria Fusion, (Becton-Dickinson) also equipped with four lasers (violet, blue, yellow-green and red) with the capacity to process two scattering and sixteen fluorescence signals (ranging between 450 to 800 nm) . This high-speed separation equipment incorporates the latest technological advances, having the ability to separate four populations simultaneously

 

3-SOFTWARE TOOLS.

 

-The Service has licenses for the use of the most common flow cytometry analysis applications  used in similar core facilities: FlowJo, Kaluza, Diva, Cytexpert and Flowlogic

 

This service is used routinely by twenty research groups. Some of the applications in analysis and / or separation that are carried out are:

-Immunophenotyping

-Characterization of transfectants

-Cell cycle and proliferation studies.

-Cellular death and viability assays (apoptosis and necrosis).

-Functional assays: ROS production, mitochondrial potential studies, Ca2 + fluxes, etc.

-Characterization of molecular associations using FRET assays.

 

                                            FARES 2021

 

 

Members

Staff Scientists
Pedro Lastres Varo
Technician
Patricia Yagüe Fernández