Field of activities which fulfill the requirements of quality standard UNE-EN ISO 9001:
Automated amino acid analysis using post column ninhydrin derivatization.
Automated protein sequencing by Edman degradation chemistry.
Since 2011 the Facility is certified according to UNE-EN ISO9001:2008 with ER-0286/2009 AENOR certification code.
The CIB Protein Chemistry Facility provides scientific and technical support to investigators from any institution, either public or private, with state-of-the-art analytical tools on the separation, identification, quantification and characterization of proteins in biological samples. Additionally, the facility provides peptide synthesis and oligonucleotide synthesis and purification.
The Protein Chemistry Facility is equipped with the following instruments:
Protein sequencer (Applied Biosystems, Procise 494). The Procise 494 Protein Sequencer performs automated Edman sequencing chemistry. Samples can be analyzed either in solution or blotted to PVDF after PAGE separation. Sensitivity is in the low pmol range.
Amino acid analyzer (Biochrom 30). The Biochrom 30 uses the classical amino acid analysis methodology based on ion-exchange liquid chromatography and postcolumn continuous reaction with ninhydrin to provide qualitative and quantitative compositional analysis. Sensitivity is ∼10 pmol.
Peptide synthesizer (AAPPtec, Focus XC). The Focus XC can synthesize up to six peptides simultaneously at scales from 0.05-5 mmol, using Fmoc chemistry.
Oligonucleotide synthesizer (Applied Biosystems 3400). The Applied Biosystems 3400 DNA Synthesizer can synthesize up to four oligonucleotides simultaneously at 40 or 200 nmol scales in less than four hours (25-mer).
HPLC (ÄKTA, Amersham Pharmacia Biotech). This instrument is dedicated to purification of peptides and proteins.
LC-MS. Mass spectrometry system (Thermo Mod. FinniganTM LXQ TM) with on line HPLC Surveyor with the following configuration:
- Pump with four separate solvent feeds (Surveyor MS pump Plus).
- Autosampler for multi sample analysis (Surveyor AS Plus).
- Photo Diode Array detector (Surveyor PDA Plus).
- Mass spectrometer LXQ equipped with linear ion trap. Mass range from 50-4000 m/z. Ionization sources: electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI).
All samples must be accompanied by a fully completed sample submission form, otherwise samples will not be processed. The forms are available on this website and can be sent electronically or preferably printed, signed and enclosed along with the sample.
Before shipping the samples, send us an email or call us to alert us for the arrival of the samples.
Please, send your samples in a padded envelope or in a box together with the submission form. The samples should be in an Eppendorf reaction tube sealed by Parafilm.
The facility is not responsible for the results obtained if the samples don´t have the suitable conditions specified in the “Sample preparation procedure” section.
AMINO ACIDS ANALYSIS
HPLC PURIFICATION (Intranet CIB)