Molecular Biology of the Chromosomes

Head of lab

We are interested in all the biological processes where DNA is involved: replication, transcription, repair and recombination, how are they regulated and how they alter and are affected by epigenetic factors such as DNA topology and chromatin organization.

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a) Initiation, Elongation, Blockage, Termination and Topology of DNA Replication.

Head:

Jorge Bernardo Schvartzman Blinder

b) Identificaction and Cloning of Genes Involved in Erythroid Differentiation.

Head:

Dora Beatriz Krimer Smunis

c) Replication Fork Barriers and DNA Instability.

Head:

Pablo Hernández Valenzuela

Staff Scientists
Pablo Hernández Valenzuela
Dora Beatriz Krimer Smunis

Ad Honorem
Jorge Bernardo Schvartzman Blinder

Junior PI
Jesus A. Carballo

Technical Staff
Alicia Rodríguez Bernabé

Technician
Marta Fernandez Díaz

Predoctoral Students
Andrea Negrin Lorenzo
Paula Alonso Ramos
Paula Alonso Ramos

Castán A., Fernández-Calleja V., Hernández P., Krimer D.B., Schvartzman J.B. and M.J. Fernández-Nestosa [2017]. Analysis of DNA topology of EBV minichromosomes in HEK 293 cells. PLoS One 12:e0188172 (2017). DOI: 10.1371/journal.pone.0188172.

Castán A., Hernández P., Krimer D.B.,Schvartzman J.B. [2017]. The abundance of Fob1 modulates de efficiency of rRFBs to stall replication forks. Nucleic Acids Res (doi: 10.1093/nar/gkx655)

Fernández-Calleja V., Hernández P., Schvartzman J.B., García-de-Lacoba, Krimer D.B [2017]. Differential gene expression analysis by RNA-seq reveals the importance of actin cytoskeletal proteins in Friend Leukemia cells. PeerJ (doi/10.7717/peerj.3432)

Penedos A, Johnson AL, Strong E, Goldman AS, Carballo JA, Cha RS [2015]. Essential and Checkpoint Functions of Budding Yeast ATM and ATR during Meiotic Prophase Are Facilitated by Differential Phosphorylation of a Meiotic Adaptor Protein, Hop1. PLoS One 10(7):e0134297. doi: 10.1371/journal.pone.0134297

Cebrián J, Castán A, Martínez V, Parra C, Kadomatsu-Hermosa MJ, Fernández-Nestosa MJ, Schaerer C, Hernández P, Krimer DB, Schvartzman JB [2015]. Direct evidence for the formation of precatenanes during DNA replication. Journal Biol Chem 290: 13725-13735.

Cebrián J, Kadomatsu-Hermosa MJ, Castán A, Martínez V, Parra, C, Fernández-Nestosa MJ, Schaerer, C, Martínez-Robles M-L, Hernández P, Krimer DB, Stasiak A, Schvartzman JB [2014]. Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules. Nucleic Acids Res (doi: 10.1093/nar/gku1255).

Cebrián J, Monturus E, Martínez-Robles M-L, Hernández P, Krimer DB, Schvartzman JB [2014]. Topoisomerase 2 Is Dispensable for the Replication and Segregation of Small Yeast Artificial Chromosomes (YACs). PLoS ONE 9(8): e104995.

Newnham L, Jordan PW, Carballo JA, Newcombe S, Hoffmann E [2013]. Ipl1/Aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis. PLoS One 8(12):e83982. doi:10.1371/journal.pone.0083982

Fernández-Nestosa MJ, Monturus E, Sánchez Z, Torres F, Fernández A, Fraga M, Hernández P, Schvartzman JB, Krimer DB [2013]. DNA methylation-mediated silencing of PU.1 in leukemia cells resistant to cell differentiation. SpringerPlus 2, 392 DOI:10.1186/2193-1801-2-392

Schvartzman JB, Martínez-Robles ML, Hernández P, Krimer DB [2013]. Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis. In Methods Mol Biol 1054, DNA Electrophoresis: Methods and Protocols (Svetlana Makovets, ed.) Springer Science Business Media, New York. DOI 10.1007/978-1-62703-565-1_7

All Publications

- MINECO, BFU2011-22489 (2012-2014)

- AECID, AP / 038170 / 11

- CSIC, I - COOP0009

- ENTRANCE

- LAB MEMBERS

- FORMER STUDENTS: What they accomplished with us and where are they now?

- MONTHLY PROGRAM

- JOURNAL CLUB

- PROTOCOLS AND LINKS

- 2D GELS

Group Leader/s

Pablo Hernández Valenzuela

Dora Beatriz Krimer Smunis

intro

Research lines

a) Initiation, Elongation, Blockage, Termination and Topology of DNA Replication.

b) Identificaction and Cloning of Genes Involved in Erythroid Differentiation.

c) Replication Fork Barriers and DNA Instability.

Members

Selected Publications

Castán A., Fernández-Calleja V., Hernández P., Krimer D.B., Schvartzman J.B. and M.J. Fernández-Nestosa [2017]. Analysis of DNA topology of EBV minichromosomes in HEK 293 cells. PLoS One 12:e0188172 (2017). DOI: 10.1371/journal.pone.0188172.

Castán A., Hernández P., Krimer D.B.,Schvartzman J.B. [2017]. The abundance of Fob1 modulates de efficiency of rRFBs to stall replication forks. Nucleic Acids Res (doi: 10.1093/nar/gkx655)

Fernández-Calleja V., Hernández P., Schvartzman J.B., García-de-Lacoba, Krimer D.B [2017]. Differential gene expression analysis by RNA-seq reveals the importance of actin cytoskeletal proteins in Friend Leukemia cells. PeerJ (doi/10.7717/peerj.3432)

Cebrián J, Castán A, Martínez V, Parra C, Kadomatsu-Hermosa MJ, Fernández-Nestosa MJ, Schaerer C, Hernández P, Krimer DB, Schvartzman JB [2015]. Direct evidence for the formation of precatenanes during DNA replication. Journal Biol Chem 290: 13725-13735.

Cebrián J, Monturus E, Martínez-Robles M-L, Hernández P, Krimer DB, Schvartzman JB [2014]. Topoisomerase 2 Is Dispensable for the Replication and Segregation of Small Yeast Artificial Chromosomes (YACs). PLoS ONE 9(8): e104995.

Newnham L, Jordan PW, Carballo JA, Newcombe S, Hoffmann E [2013]. Ipl1/Aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis. PLoS One 8(12):e83982. doi:10.1371/journal.pone.0083982

Fernández-Nestosa MJ, Monturus E, Sánchez Z, Torres F, Fernández A, Fraga M, Hernández P, Schvartzman JB, Krimer DB [2013]. DNA methylation-mediated silencing of PU.1 in leukemia cells resistant to cell differentiation. SpringerPlus 2, 392 DOI:10.1186/2193-1801-2-392

Funding

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