Proteins are effectors of most physiological functions in cells and they play a key role in the extracellular medium. Protein function is finely tuned at multiple levels including synthesis and degradation, intracellular localization and posttranslational modifications. The posttranslational modification of proteins is a key mechanism for the regulation of their biological activity. Various biological mediators, including lipids and reactive oxygen and nitrogen species, and also some drugs and toxins, can modify proteins posttranslationally. We are interested in the study of the importance of these processes in molecular pathophysiology and in the mechanisms of drug action. Our work includes several aspects: the structural and functional characterization of novel types of posttranslational modifications, the identification of novel protein targets for modification and the study of the pathophysiological consequences of these phenomena, mainly in the context of inflammatory and oncogenic processes.
Díez-Dacal B, Sánchez-Gómez FJ, Sánchez-Murcia PA, Milackova I, Zimmerman T, Ballekova J, García-Martín E, Agúndez JA, Gharbi S, Gago F, Stefek M, Pérez-Sala D . Molecular interactions and implications of aldose reductase inhibition by PGA1 and clinically used prostaglandins. Mol Pharmacol. 89:42-52
Sánchez-Gómez FJ, González-Morena J, Vida Y, Pérez-Inestrosa E, Blanca M, Torres MJ, Pérez-Sala D . Amoxicillin haptenates intracellular proteins that can be transported in exosomes to target cells. Allergy. DOI: 10.1111/all.12958
Klionsky D,... Oeste CL,...Pérez-Sala D,... et al. . Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 12:1-222
Sánchez-Gómez FJ, Díez-Dacal B, García-Martín E, Agúndez JA, Pajares MA, Pérez-Sala D . Detoxifying Enzymes at the cross-roads of inflammation, oxidative stress, and drug hypersensitivity: Role of glutathione transferase P1-1 and aldose reductase. Front Pharmacol. 7:237.
Nuin E, Pérez-Sala D, Lhiaubet-Vallet V, Andreu I, Miranda MA . Photosensitivity to Triflusal: Formation of a Photoadduct with Ubiquitin Demonstrated by Photophysical and Proteomic Techniques. Front Pharmacol. doi: 10.3389/fphar.2016.00277
Pérez-Sala D, Oeste CL, Martínez AE, Carrasco, MJ, Garzón B, Cañada FJ . Vimentin filament organization and stress sensing depend on its single cysteine residue and zinc binding. Nat Commun 6:7287
Pérez-Sala D, Oeste CL, Sánchez-Gómez FJ . Vimentin gets a new glow from zinc. Oncotarget 6:15742-15743
Ariza A, Mayorga C, Fernández TD, Barbero N, Martín-Serrano A, Pérez-Sala D, Sánchez-Gómez FJ, Blanca M, Torres MJ, Montañez MI . Hypersensitivity reactions to beta-lactams: Relevance of hapten-protein conjugates. J Allergol Clin Immunol 25:12-25
Aldini G, Domingues RM, Spickett CM, Domingues P, Altomare A, Sánchez-Gómez FJ, Oeste CL, Pérez-Sala D . Protein lipoxidation: Detection strategies and challenges. Redox Biol 5:253-266
EU Project 675132 (H2020-MSCA-ITN-2015). Innovative Training Network. "MASS Spectrometry TRaining network for Protein Lipid adduct Analysis". MASSTRPLAN. Oct 2015-Sept 2019
"Protein modification by lipoxidation and drug addition: novel perspectives for exploring disease mechanisms and therapeutic strategies" MINECO SAF2015-68590-R (cofunded FEDER).
Network for the Research on Adverse Reactions to Drugs and Allergens. Instituto de Salud Carlos III. RETIC RD12/0013/0008
COST Action CA15214 EUROCELLNET "An integrative action for multidisciplinary studies on cellular structural networks"
COST Action CM1001 "Chemistry of non-enzymatic protein modification: modulation of protein structure and function"
Acción COST (European Cooperation in the field of Scientific and Technical Research): Action TD1304 Zinc-Net: the Network for the Biology of Zinc
- "Protein tag for endo-lysosomal localization and degradation". D. Pérez-Sala, P. Boya, K, Stamatakis. P200802721. September 25, 2008.
- "Compounds with 2-cyclopentenone structure as inhibitors of ALR family enzymes". D. Pérez-Sala, B. Díez. P201030449. March 25, 2010.
- EU COST Action CM1001: Chemistry of non-enzymatic protein modification- modulation of protein structure and function. http://users.unimi.it/cm1001/
- Red de Reacciones Adversas a Alergenos y Fármacos (RIRAAF) RETIC ISCIII. https://chirimoyo.ac.uma.es/riraaf/