T lymphocyte activation

T lymphocyte activation and differentiation involves antigen recognition plus interaction between costimulatory molecules and their ligands in myeloid cells. These interactions deliver signals that are essential to mount immune responses against pathogens and cancer, or to develop autoimmune diseases. Our interest is focused on the role of CD28, the CD28 homolog molecule ICOS (CD278), the ICOS ligand (ICOS-L, B7h, CD275), and PI3K signals in T lymphocyte, NK cell and antigen presenting cells in vitro or in vivo using pharmacologic and genetic approaches. We are also interested in the use and impact of nanomaterials to modulate innate and adaptive immunity.

Staff Scientists
José María Rojo Hernández

Rojo, José M., Montes-Casado, María, Aragoneses-Fenoll, Laura, Ojeda, Gloria, Dianzani, Umberto, Portolés, Pilar [2021]. PI3-Kinase p110α Deficiency Modulates T Cell Homeostasis and Function and Attenuates Experimental Allergic Encephalitis in Mature Mice. International Journal of Molecular Sciences. 22:-.

Estrada-Capetillo L, Aragoneses-Fenoll L, Domínguez-Soto A, Fuentelsaz-Romero S, Nieto C, Simón-Fuentes M, Alonso B, Portolés P, Corbí AL, Rojo JM, Puig-Kröger A [2021]. CD28 is expressed by macrophages with anti-inflammatory potential and limits their T cell activating capacity. European Journal of Immunology. 51: 824-834. doi: 10.1002/eji.202048806

Montes-Casado M, Sanvicente A, Casarrubios L, Feito MJ, Rojo JM, Vallet-Regí M, Arcos D, Portolés P, Portolés MT [2020]. An immunological approach to the biocompatibility of mesoporous SiO2-CaO nanospheres. International Journal of Molecular Sciences. 21: 8291; doi:10.3390/ijms21218291

Raineri D, Dianzani C, Cappellano G, Maione F, Baldanzi G, Iacobucci I, Clemente N, Baldone G, Boggio E, Gigliotti CL, Boldorini R, Rojo JM, Monti M, Birolo L, Dianzani U, Chiocchetti A, [2020]. Osteopontin binds ICOSL promoting tumor metastasis. Communications Biology. 3: 615. doi: https://doi.org/10.1038/s42003-020-01333-1 |

Clemente N, Boggio E, Gigliotti LC, Raineri D, Ferrara B, Miglio G, Argenziano M, Chiocchetti A, Cappellano G, Trotta F, Caldera F, Capucchio MT, Yagi J, Rojo JM, Renò F, Cavalli R, Dianzani C, Dianzani U [2020]. Immunotherapy of experimental melanoma with ICOS-Fc loaded in biocompatible and biodegradable nanoparticles. Journal of Controlled Release 320: 112-124. doi: https://doi.org/10.1016/j.jconrel.2020.01.030

Montes-Casado M, Ojeda G, Aragoneses-Fenoll L, López D, de Andrés B, Gaspar ML, Dianzani U, Rojo JM, Portolés P [2019]. ICOS deficiency hampers the homeostasis, development and function of NK cells. PloS ONE: 14:e0219449. doi: https://doi.org/10.1371/journal.pone.0219449

Feito MJ, Diez-Orejas R, Cicuéndez M, Casarrubios L, Rojo JM, Portolés MT [2019]. Characterization of M1 and M2 polarization phenotypes in peritoneal macrophages after treatment with graphene oxide nanosheets. Colloids Surf. B: Biointerfaces 176: 96-105. doi: https://doi.org/10.1016/j.colsurfb.2018.12.063

Diez-Orejas R, Feito MJ, Cicuéndez M, Casarrubios L, Rojo JM, Portolés MT [2018]. Graphene oxide nanosheets increase Candida albicans killing by pro-inflammatory and reparative peritoneal macrophages. Colloids Surf. B: Biointerfaces 171:250-259. doi: https://doi.org/10.1016/j.colsurfb.2018.07.027

Aragoneses-Fenoll L, Ojeda G, Montes-Casado M, Acosta-Ampudia Y, Dianzani U, Portolés P, Rojo JM
[2018]. "T-cell specific loss of the PI-3 kinase p110a catalytic subunit results in enhanced cytokine production and anti tumor response" Frontiers in Immunology. 9:332. doi: 10.3389/fimmu.2018.00332

Díez-Orejas R, Feito MJ, Cicuéndez M, Rojo JM, Portolés MT
[2018]. "Differential effects of graphene oxide nanosheets on Candida albicans phagocytosis by murine peritoneal macrophages" Journal of Colloid and Interface Science. 512: 665-673. doi: https://doi.org/10.1016/j.jcis.2017.10.104

All Publications

Grant SAF2017-89672-R: “Lipid rafts y entorno tumoral inflamatorio en la terapia del cáncer: Análogos alquilfosfolípidos como agentes dirigidos frente a lipid rafts e inflamación con múltiples indicaciones terapéuticas”. Agencia Estatal de Investigación.
PI: Faustino Mollinedo (01/2018-12/2020)

Grant PI13/01809: “ICOS, ICOS ligand and Class IA PI3-kinases as targets in immunotherapy". Acción Estratégica en Salud, IS Carlos III (01/2014-09/2017)

Grant PI10/00650: “ICOS signaling in costimulation and adhesion, conditioned deficiency in T lymphocyte lineages, and impact in autoimmune inflammatory diseases” AES, IS Carlos III (01/2011-12/2013)

Grant PI070620: “Mechanisms of activation by ICOS (CD278): Pro- and anti-inflammatory effects in Experimental Allergic Encephalitis” Fondo de Investigación Sanitaria, IS Carlos III (11/2007-11/2010)

Grant SAF2004-06852, "Inducible Costimulator (ICOS): Integration of costimulatory pathways in T lymphocytes and specific signaling" MEC, Programa Nacional de Salud y Farmacia (12/2004-12/2007).

Grant BMC2001-2177, “Variability mechanisms of “invariant” CD3ε chains of T lymphocyte TCR/CD3 complex: Changes in complex structure and activation signals" MCyT, Programa Nacional de Promoción General del Conocimiento (12/ 2001-12/2004)

PhD Thesis

Acosta Ampudia YY (2013) "Association of Class IA PI3-kinase subunits to ICOS (CD278) and relevance to costimulation" PhD Thesis, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid. Director: Rojo, JM. Summa “cum laude”.

Bello R (2007) "Immunological and functional analysis of CD3ε chains of the TCR/CD3 complex". PhD Thesis, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid. Director: Rojo, JM. Summa “cum laude”.

Sánchez, A (2006) "Analysis of costimulatory effect of CD46 (MCP) in CD4+ T lymphocytes. Interaction with pathogens and search of potential ligands". PhD Thesis, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid. Director: Rojo, JM. Summa “cum laude”.

Group Leader/s

José María Rojo Hernández

intro

Research lines

Mechanisms of T lymphocyte costimulation by "Inducible Costimulator" (ICOS, CD278)

Members

Selected Publications

Montes-Casado M, Sanvicente A, Casarrubios L, Feito MJ, Rojo JM, Vallet-Regí M, Arcos D, Portolés P, Portolés MT [2020]. An immunological approach to the biocompatibility of mesoporous SiO2-CaO nanospheres. International Journal of Molecular Sciences. 21: 8291; doi:10.3390/ijms21218291

Montes-Casado M, Ojeda G, Aragoneses-Fenoll L, López D, de Andrés B, Gaspar ML, Dianzani U, Rojo JM, Portolés P [2019]. ICOS deficiency hampers the homeostasis, development and function of NK cells. PloS ONE: 14:e0219449. doi: https://doi.org/10.1371/journal.pone.0219449

Diez-Orejas R, Feito MJ, Cicuéndez M, Casarrubios L, Rojo JM, Portolés MT [2018]. Graphene oxide nanosheets increase Candida albicans killing by pro-inflammatory and reparative peritoneal macrophages. Colloids Surf. B: Biointerfaces 171:250-259. doi: https://doi.org/10.1016/j.colsurfb.2018.07.027

Aragoneses-Fenoll L, Ojeda G, Montes-Casado M, Acosta-Ampudia Y, Dianzani U, Portolés P, Rojo JM
[2018]. "T-cell specific loss of the PI-3 kinase p110a catalytic subunit results in enhanced cytokine production and anti tumor response" Frontiers in Immunology. 9:332. doi: 10.3389/fimmu.2018.00332

Díez-Orejas R, Feito MJ, Cicuéndez M, Rojo JM, Portolés MT
[2018]. "Differential effects of graphene oxide nanosheets on Candida albicans phagocytosis by murine peritoneal macrophages" Journal of Colloid and Interface Science. 512: 665-673. doi: https://doi.org/10.1016/j.jcis.2017.10.104

Funding

More info

Group Leader/s

José María Rojo Hernández

intro

Research lines

Mechanisms of T lymphocyte costimulation by "Inducible Costimulator" (ICOS, CD278)

Members

Selected Publications

Montes-Casado M, Sanvicente A, Casarrubios L, Feito MJ, Rojo JM, Vallet-Regí M, Arcos D, Portolés P, Portolés MT [2020]. An immunological approach to the biocompatibility of mesoporous SiO2-CaO nanospheres. International Journal of Molecular Sciences. 21: 8291; doi:10.3390/ijms21218291

Montes-Casado M, Ojeda G, Aragoneses-Fenoll L, López D, de Andrés B, Gaspar ML, Dianzani U, Rojo JM, Portolés P [2019]. ICOS deficiency hampers the homeostasis, development and function of NK cells. PloS ONE: 14:e0219449. doi: https://doi.org/10.1371/journal.pone.0219449

Diez-Orejas R, Feito MJ, Cicuéndez M, Casarrubios L, Rojo JM, Portolés MT [2018]. Graphene oxide nanosheets increase Candida albicans killing by pro-inflammatory and reparative peritoneal macrophages. Colloids Surf. B: Biointerfaces 171:250-259. doi: https://doi.org/10.1016/j.colsurfb.2018.07.027

Aragoneses-Fenoll L, Ojeda G, Montes-Casado M, Acosta-Ampudia Y, Dianzani U, Portolés P, Rojo JM [2018]. "T-cell specific loss of the PI-3 kinase p110a catalytic subunit results in enhanced cytokine production and anti tumor response" Frontiers in Immunology. 9:332. doi: 10.3389/fimmu.2018.00332

Díez-Orejas R, Feito MJ, Cicuéndez M, Rojo JM, Portolés MT [2018]. "Differential effects of graphene oxide nanosheets on Candida albicans phagocytosis by murine peritoneal macrophages" Journal of Colloid and Interface Science. 512: 665-673. doi: https://doi.org/10.1016/j.jcis.2017.10.104

Funding

More info

Aragoneses-Fenoll L, Ojeda G, Montes-Casado M, Acosta-Ampudia Y, Dianzani U, Portolés P, Rojo JM
[2018]. "T-cell specific loss of the PI-3 kinase p110a catalytic subunit results in enhanced cytokine production and anti tumor response" Frontiers in Immunology. 9:332. doi: 10.3389/fimmu.2018.00332

Díez-Orejas R, Feito MJ, Cicuéndez M, Rojo JM, Portolés MT
[2018]. "Differential effects of graphene oxide nanosheets on Candida albicans phagocytosis by murine peritoneal macrophages" Journal of Colloid and Interface Science. 512: 665-673. doi: https://doi.org/10.1016/j.jcis.2017.10.104