Description

In collaboration with the groups of Dr. Miguel Ángel Pardo (Centro tecnológico AZTI-Tecnalia, Derio) and of Prof. Giuseppe Spano (University of Foggia, Italia), we have been able to detect the colonization of the gut of zebrafish by probiotic bacteria carrying vectors that constitutively express mCherry protein. This methodology has been also utilized to analyze the colonización of mice gut by probiotic bacteria in collaboration with Dr. Jean Guy LeBlanc (Centro de Referencia de Lactobacilos, tucuman, Argentina). This research falls within the CYTED strategic project 917PTE0537  and PCIN-2017-075.

Also, we have studied the capacity of pneumococcus to adhere to human lung epithelial cells, as well as the phagocytosis of this pathogen by macrophages and neutrophiles,  using the maltose inducible gpf vector.

We are continuing this line of research by standardizing quantitative evaluation methodology based on fluorescence  for further studies on bacterial colonization both in vivo in zebrafish and mice models also in in vitro interactomic studies on bacterial cells (pathogens or probiotic) and eucaryotic cell lines.

References:

Russo P., Iturria I., Mohedano M.L., Caggianiello G., Rainieri S., Fiocco D., Pardo M. A., López P. and Spano G. (2015). Zebrafish gut colonization by mCherry-labelled lactic acid bacteria. Appl. Microbiol. Biotechnol. 99:3479-3490.

Nácher-Vázquez, M., Ballesteros, N., Canales, A., Rodríguez Saint-Jean, S., Pérez-Prieto, S.I., Prieto, A., Aznar, R. and López, P. (2015). Dextrans produced by lactic acid bacteria exhibit antiviral and immunomodulatory activity against salmonid viruses. Carbohy. Polym. 124:292–301.

Zarour, K., Prieto, A., Pérez-Ramos, A., Kihal, M. and López, P. (2018). Analysis of technological and probiotic properties of Algerian L. mesenteroides strains isolated from dairy and non-dairy products. J. Funct. Foods 49:351-361.

Hernández-Alcántara, A.M., Wacher, C., Llamas, M.G., López, P., Pérez-Chabela, M.L. (2018). Probiotic properties and stress response of thermotolerant lactic acid bacteria isolated from cooked meat products. LWT - Food Sci. Tech. 91:249-257.

Pérez-Ramos, A., Mohedano, M.L., Pardo, M.A. and López, P. (2018). β-glucan-producing Pediococcus parvulus 2.6: test of probiotic and immunomodulatory properties in zebrafish models. Front. Microbiol. 9:1684. 

Llamas-Arriba, M.G., Peirotén, A., Puertas, A.I., Prieto, A., López, P., Pardo, M.A., Rodríguez, E., Dueñas, M.T. (2019). Heteropolysaccharide-producing bifidobacteria for the development of functional dairy products. LWT Food Sci Technol. 102:295-303. 

Mohedano, M.L., Hernández-Recio, S., Yépez, A., Requena, T., Martínez-Cuesta, M.C., Peláez, C., Russo, P., LeBlanc, J.G., Spano G, Aznar, R. and López, P. (2019). Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling. Front. Microbiol. 10:1748.

Besrour-Aouam, N., Mohedano, M.L., Fhoula, I., Zarour, K., Najjari, A., Aznar, R., Prieto, A., Ouzari, H.-I. López, P. (2019). Different Modes of regulation of the expression of dextransucrase in Leuconostoc lactis AV1n and Lactobacillus sakei MN1. Front. Microbiol. 10:959.

Hernández-Alcántara, A., Pardo, S., Mohedano. M.L., Vignolo, G, de Moreno de LeBlanc, A., LeBlanc, J.G., Aznar, R. and López, P. (2020). The ability of riboflavin-overproducing Lactiplantibacillus plantarum strains to survive under gastrointestinal conditions. Front. Microbiol. 11:591945.