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  2. Protein engineering against antimicrobial resistance
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Hermoso JA, García JL, García P  [2007]. Taking aim on bacterial pathogens: from phage therapy to enzybiotics. Curr Opin Microbiol 10:461-472

Rodríguez-Cerrato V, García P, del Prado G, García E, Gracia M, Huelves L, Ponte C, López R, Soriano F  [2007]. In vitro interactions of LytA, the major pneumococcal autolysin, with two bacteriophage lytic enzymes (Cpl-1 and Pal), cefotaxime, and moxifloxacin against antibiotic-susceptible and -resistant.... J Antimicrob Chemother 60:1159-1162

Molina R, González A, Moscoso M, García P, Stelter M, Kahn R, Hermoso JA  [2007]. Crystallization and preliminary X-ray diffraction studies of choline-binding protein F from Streptococcus pneumoniae. Acta Cryst F 63:742-745

Pérez-Dorado I, Campillo NE, Monterroso B, Hesek D, Lee M, Páez JA, García P, Martínez-Ripoll M, García JL, Mobashery S, Menéndez M, Hermoso JA  [2007]. Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1. J. Biol. Chem. 282:24990-24999

Rodríguez-Cerrato V, García P, Huelves L, García E, del Prado G, Gracia M, Ponte C, López R, Soriano F  [2007]. Pneumococcal LytA autolysin, a potent therapeutic agent in experimental peritonitis-sepsis caused by highly beta-lactam-resistant Streptococcus pneumoniae. Antimicrob Agents Chemother 51:3371-3373

Romero P, López R, García E  [2007]. Key role of amino acid residues in the dimerization and catalytic activation of the autolysin LytA, an important virulence factor in Streptococcus pneumoniae. J Biol Chem 282:17729-17737

Llull D, Rivas L, García E  [2007]. In vitro bactericidal activity of the antiprotozoal drug miltefosine against Streptococcus pneumoniae and other pathogenic streptococci. Antimicrob Agents Chemother 51:1844-1848

de Eugenio LI, García P, Luengo JM, Sanz JM, San Román J, García JL, Prieto MA  [2007]. Biochemical evidence the phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442. Characterization of a paradigmatic enzyme.. J Biol Chem 282:4951-4962

Romero P, Llull D, García E, Mitchell TJ, López R, Moscoso M  [2007]. Isolation and characterization of a new plasmid pSpnP1 from a multidrug-resistant clone of Streptococcus pneumoniae. Plasmid 58:51-60

Maestro B, González A, García P, Sanz JM  [2007]. Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines. FEBS J 274:364-376

López R  [2006]. Pneumococcus: the sugar-coated bacteria. Int Microbiol 9:179-190

Balsalobre L, Hernández-Madrid A, Llull D, Martín-Galiano AJ, García E, Fenoll A, G. de la Campa A  [2006]. Molecular characterization of disease-associated streptococci of the mitis group that are optochin susceptible. J Clin Microbiol 44: 4163–4171

Moscoso M, García E, López R  [2006]. Biofilm formation by Streptococcus pneumoniae: role of choline, extracellular DNA, and capsular polysaccharide in microbial accretion. J Bacteriol 188:7785-7795

Llull D, López R, García E  [2006]. Skl, a novel choline-binding N-acetylmuramoyl-L-alanine amidase of Streptococcus mitis SK137 containing a CHAP domain. FEBS Lett 580:1959-1964

Moldes C, Farinós GP, de Eugenio LI, García P, García JL, Ortego F, Hernández-Crespo P, Castañera P, Prieto MA  [2006]. New tool for spreading proteins to the environment: Cry1Ab toxin immobilized to bioplastics. Appl Microbiol Biotechnol 72:88-93

Llull D, López R, García E  [2006]. Characteristic signatures of the lytA gene provide a rapid and reliable diagnosis of Streptococcus pneumoniae infections. J Clin Microbiol 44:1250-1256

Houston KM, Egan CA, García P, Harnett W.  [2006]. Investigation of strategies with potential for producing a phosphorylcholine-free version of the filarial nematode immunomodulator, ES-62. J Helminthol 80:261-265

Lagartera L, González A, Hermoso JA, Sáiz JL, García P, García JL, Menéndez M  [2005]. Pneumococcal phosphorylcholine esterase, Pce, contains a metal binuclear center that is essential for substrate binding and catalysis. Prot Sci 14:3013-3024

Moscoso M, Obregón V, López R, García JL, García E  [2005]. Allelic variation of the polymorphic locus lytB, encoding a choline-binding protein, from streptococci of the mitis group. Appl Environ Microbiol 71:8706-8713

García P, García J L, López R, García E  [2005]. Pneumococcal phages. Eds: Waldor MK, Friedman DI, Adhya SL. Phages: Their Role in Bacterial Pathogenesis and Biotechnology. ASM Press, Washington, DC, pp 335-361

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