We are interested in the study of the function of the Polycomb group (PcG) of genes, an evolutionary conserved system involved in the maintenance of transcriptionally repressed states relevant to cell identity. The products of the PcG genes form multiprotein complexes whose activity in transcriptional control is mediated, at least in part, by the histone modifier activities of some of their subunits. In addition to their function during embryogenesis, Polycomb complexes play roles in adult stem cells relevant to normal tissue regeneration and cancer. The lab is focused on the function of Polycomb complexes containig Ring1A and Ring1B, the E3 ligases involved in monoubiquitylation of histone H2A.
Vidal, M. and Starowicz, K. . Polycomb complexes PRC1 and their function in hematopoiesis Exp. Hematol. 48:1231
Ikawa,T., Masuda, K., Endo, T.A., Endo,M., Isono, K., Koseki, Y., Nakagawa, R., Kometani, K., Takano,Y., Agata, Y., Katsura, Y., Kurosaki, T.,Vidal, M., Koseki, H. and Kawamoto, H. . Conversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program. Genes & Dev. 30:2475-2485
Calés C, Pavón L, Starowicz K, Pérez C, Bravo M, Ikawa T, Koseki H and Vidal M. . Role of Polycomb RYBP in Maintaining the B-1 to B-2 B-Cell Lineage Switch in Adult Hematopoyesis. Mol. Cell. Biol. 36:900-912
Yakushiji-Kaminatsui N, Kondo T, Endo TA, Koseki Y, Kondo K, Ohara O, Vidal M, Koseki H. . RING1 contributes to early proximal-distal specification of the forelimb bud by restricting Meis2 expression. Development 143:276-285
Bravo M, Nicolini F, Starowicz K, Barroso S, Calés C, Aguilera A, Vidal M . Polycomb RING1A- and RING1B-dependent histone H2A monoubiquitylation at pericentromeric regions promotes S-phase progression. J. Cell Sci. 128:3660-71.
Morimoto-Suzki N, Hirabayashi Y, Tyssowski K, Shinga J, Vidal M, Koseki H, Gotoh Y . The polycomb component Ring1B regulates the timed termination of subcerebral projection neuron production during neocortical development. Development 141:4343-53
Kondo T, Isono K, Kondo K, Endo TA, Itohara S, Vidal M, Koseki H . Polycomb potentiates Meis2 activation in midbrain by mediating interaction of the promoter with a tissue-specific enhancer. Dev. Cell 28:94-101.
Vidal M . Polycomb complexes: chromatin regulators required for cell diversity and tissue homeostasis, in Transcriptional and Epigenetic Mechanisms Regulating Normal and Aberrant Blood Cell Development, Epigenetics and Human Health. Springer pp 95-139
Martínez-Gómez AI, Villegas S, Aguado-Llera C, Bacarizo J, Cámara-Artigas A, Vidal M, Neira JL . The isolated N terminus of Ring1B is a well-folded, monomeric fragment with native-like structure. Protein Eng. Des. Sel. 27:1-11
Fundación Ramón Areces (2010-2012)